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file:Figure-3.jpg|{{figure number|3}}A shale sample imaged using SE1 signal (left) and SE2 signal (right). Surface-specific information such as pore space and surface roughness is evident in the SE1 image. The SE2 image has more compositional influence, displaying organic matter (OM) bodies that are not evident in the SE1 image.<ref name=Huangetal_2013 />
 
file:Figure-3.jpg|{{figure number|3}}A shale sample imaged using SE1 signal (left) and SE2 signal (right). Surface-specific information such as pore space and surface roughness is evident in the SE1 image. The SE2 image has more compositional influence, displaying organic matter (OM) bodies that are not evident in the SE1 image.<ref name=Huangetal_2013 />
file:M102Ch1Fg4.jpg|{{figure number|4}}SE2 (a) and BSE1 (b) image of a cross section of a shale rock. Note that the contrast between carbonate (Ca) and silica (SiO<sub>2</sub>) grains is much higher in BSE1; the topographical information is greater in the SE2 image (OM-associated nanopores are not visible in BSE1).<ref name=Huangetal_2013 />
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file:M102Ch1Fg4.jpg|{{figure number|4}}SE2 (a) and BSE1 (b) image of a cross section of a shale rock. Note that the contrast between carbonate (ca) and silica (si) grains is much higher in BSE1; the topographical information is greater in the SE2 image (OM-associated nanopores are not visible in BSE1).<ref name=Huangetal_2013 />
 
file:M102Ch1Fg5.jpg|{{figure number|5}}A BSE2 image of gold (Au) nanoparticles showing crystallographic contrast.<ref name=Huangetal_2013 />]]
 
file:M102Ch1Fg5.jpg|{{figure number|5}}A BSE2 image of gold (Au) nanoparticles showing crystallographic contrast.<ref name=Huangetal_2013 />]]
 
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[[:file:M102Ch1Fg4.jpg|Figure 4]]a shows an SE2 image of a cross section of a shale sample that has been polished by argon-ion milling, a sample preparation technique that consists of using one or more beams of argon ions to gently polish the surface of a sample by sputtering away material, thus providing an extremely smooth surface for SEM investigation. The image was acquired with an Everhart-Thornley type secondary electron detector.
 
[[:file:M102Ch1Fg4.jpg|Figure 4]]a shows an SE2 image of a cross section of a shale sample that has been polished by argon-ion milling, a sample preparation technique that consists of using one or more beams of argon ions to gently polish the surface of a sample by sputtering away material, thus providing an extremely smooth surface for SEM investigation. The image was acquired with an Everhart-Thornley type secondary electron detector.
The SE2 image contrast reveals both topographical and compositional information due to the greater sample interaction depth of SE2 electrons. High SE yield is scaled as lighter shades of gray, and low SE yield is scaled as dark shades of gray in SEM images. Pores and fractures appear dark in the SE2 image, reflecting lower secondary electron yield from negative depressions than from elsewhere on the sample surface. Therefore, porosity information can be readily characterized with the SE2 electrons.
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The SE2 image contrast reveals both topographical and compositional information due to the greater sample interaction depth of SE2 electrons. High SE yield is scaled as lighter shades of gray, and low SE yield is scaled as dark shades of gray in SEM images. Pores and [[fracture]]s appear dark in the SE2 image, reflecting lower secondary electron yield from negative depressions than from elsewhere on the sample surface. Therefore, [[porosity]] information can be readily characterized with the SE2 electrons.
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The same cross section was imaged with BSE1 with the Energy-selective backscatter (EsB) detector (Figure 4b). The image contrast (grayscale variation) reflects compositional variations (mean atomic number) of the sample. For example, the midgray represents silica matrix; the darker level represents organic matter. The brighter gray level reflects higher density carbonate phases, and the brightest gray level represents pyrite. Note the greater compositional contrast provided by the BS1 image (Figure 4b) over the SE2 image (Figure 4a). Although SE2 and BS1 images are capable of providing compositional information, auxiliary techniques, such as energy-dispersive x-ray spectrometry (EDS), are required to characterize elemental composition.
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The same cross section was imaged with BSE1 with the energy-selective backscatter (EsB) detector (Figure 4b). The image contrast (grayscale variation) reflects compositional variations (mean atomic number) of the sample. For example, the midgray represents [[silica]] matrix; the darker level represents organic matter. The brighter gray level reflects higher density [[carbonate]] phases, and the brightest gray level represents [[pyrite]]. Note the greater compositional contrast provided by the BS1 image (Figure 4b) over the SE2 image (Figure 4a). Although SE2 and BS1 images are capable of providing compositional information, auxiliary techniques, such as energy-dispersive x-ray spectrometry (EDS), are required to characterize elemental composition.
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Compared to BSE1, for which the contrast is modulated by mean atomic number differences, BSE2 yield depends strongly on crystalline structures such as grain orientations. [[:file:M102Ch1Fg5.jpg|Figure 5]] shows a BSE2 image of gold (Au) nanoparticles. Contrast corresponding to different grains is revealed in the image despite all the chemically identical grains. Therefore, BSE2 electrons can be used to image crystallographic contrast in polycrystalline materials.
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Compared to BSE1, for which the contrast is modulated by mean atomic number differences, BSE2 yield depends strongly on crystalline structures such as grain orientations. [[:file:M102Ch1Fg5.jpg|Figure 5]] shows a BSE2 image of [[gold]] (Au) nanoparticles. Contrast corresponding to different grains is revealed in the image despite all the chemically identical grains. Therefore, BSE2 electrons can be used to image crystallographic contrast in polycrystalline materials.
    
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